Chemiluminescence immunoassay for somatomedin C in serum.
نویسندگان
چکیده
To select the best tracer for use in a competitive immunoassay, we conjugated human somatomedin C (SmC) to various chemiluminescent compounds via two different synthetic pathways. Naphthylhydrazides and arylhydrazides, used as the labels, were incorporated via their imidate or their succinimide esters. Conjugating the carboxy terminal of (amino ethyl)ethyl-isoluminol to SmC via a succinimide linkage supplied the most sensitive detection limit and the most immunoreactive conjugate. We developed an immunoassay based on the use of this conjugate, and evaluated dextran-coated charcoal, second-antibody precipitation, and solid-phase immunoprecipitation for separating bound and free label. This chemiluminescent method has a detection limit of 16 pg per tube, and it is accurate and precise. Correlation studies with a conventional radioimmunoassay (x) for SmC gave the following regression equation: y = 0.66x + 3.76 (r = 0.953, n = 30); the slight discrepancies between the two methods are probably ascribable to the use of different antibodies. We thus propose this chemiluminescence immunoassay as an inexpensive and sensitive alternative to radioimmunoassay for measuring SmC in serum or in extracts of serum.
منابع مشابه
Serum insulin-like growth factor-1 levels in females and males in different cervical vertebral maturation stages
OBJECTIVE The aim of this cross sectional study was to assess serum insulin-like growth factor-1 (IGF-1) levels in female and male subjects at various cervical vertebral maturation (CVM) stages. MATERIAL AND METHODS The study sample consisted of 60 subjects, 30 females and 30 males, in the age range of 8-23 years. For all subjects, serum IGF-1 level was estimated from blood samples by means o...
متن کاملSerum IGF-1 levels as a clinical tool for optimizing orthodontic treatment timing
BACKGROUND The study aims to associate serum insulin-like growth factor-1 (IGF-1) levels with cervical maturation stages (CS) 3, 4, and 5 on lateral cephalogram in male subjects and to find out peak serum IGF-1 levels among these three stages. METHODS The study was conducted on 45 male subjects who were at skeletal maturation stage CS-3, CS-4, and CS-5. Subjects were selected using simple ran...
متن کاملEnzyme Immunoassay for the Quantitative Determination of TESTOSTERONE in Human Serum or plasma INTRODUCTION OF CHEMILUMINESCENCE IMMUNOASSAY
متن کامل
Direct chemiluminescence immunoassay for estradiol in serum.
In this simple, reliable, fast solid-phase chemiluminescence immunoassay for directly measuring (i.e., without prior extraction) estradiol-17 beta in serum, a monoclonal antibody is used that binds estradiol with high affinity (Ka = 10(10) L/mol), and does not bind other steroids tested, the highest cross reactivity observed being 0.1% for estradiol-17 alpha. In this system the monoclonal antib...
متن کاملEvaluation of assay methods and false positive results in the laboratory diagnosis of hepatitis C virus infection
Diagnosis of hepatitis C virus (HCV) infection routinely performed in the microbiology laboratories is based on the detection of serum antibodies against hepatitis C virus (anti-HCV) by two main methods: enzyme immunoassay (EIA) and chemiluminescence immunoassay (CIA). Different reactives built on these two methods were manufactured and are still in use for the diagnosis of HCV infections. The ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Clinical chemistry
دوره 33 11 شماره
صفحات -
تاریخ انتشار 1987